A. Typical Cruise Chemical Inventory
|
Common Name of Material |
Qty |
Notes |
Trained Individual |
Spill control |
|
Ethyl alcohol (95%) |
80L (in 20L cans) |
UN1170, Waste contained and disposed of by SWFSC at end of project. Stored in fume hood |
Bryan Overcash, Amy Hays |
F |
|
Formaldehyde solution (37%) |
20L |
No waste. Stored in fume hood |
Bryan Overcash, Amy Hays |
F |
|
Tris buffer |
500ml |
Stored in Chem lab |
Bryan Overcash, Amy Hays |
F |
|
Sodium borate powder |
500gr |
Stored in Chem lab |
Bryan Overcash, Amy Hays |
F |
|
HCL (1.2N) |
4L |
UN1789, No waste. Stored in chem lab. |
David Wolgast |
A |
|
Sulfuric acid (10 Normal) |
4L |
Stored in Chem lab, waste neutralized by base in assay |
David Wolgast |
A |
|
Acetone (90%) |
7L |
UN1090, Waste contained and disposed of by SIO at end of project. Stored chem locker. |
David Wolgast |
F |
|
Mangannous Chloride |
4L |
No waste. stored in chem locker |
David Wolgast |
A |
|
Sodium Hydroxide/Sodium Iodide |
4L |
UN1824, Waste neutralized by acid in assay. Stored in chem locker |
David Wolgast |
A |
|
Ethanol (95%) |
1L |
UN1170, No waste. Stored in Constant environment room |
David Wolgast |
F |
|
Ecolume Scintillation Fluid |
2.5L |
No waste. Stored chem locker. |
David Wolgast |
F |
|
HCL (12N) |
150ml |
No waste. Stored in wet lab/Dropper bottles with secondary containment |
Shonna Dovel |
A |
|
Isopropyl Alcohol (91%) |
30ml |
No waste. Stored in wet lab/Dropper bottles with secondary containment |
Shonna Dovel |
A |
|
Liquid Nitrogen |
50L Dewar |
No waste. Stored wet lab |
Shonna Dovel |
A |
|
Acetone (90%) |
7L |
No waste. Stored in wet lab and -80 freezer with secondary containment |
Shonna Dovel |
F |
|
HCL (1N) |
400ml |
No waste. Stored in wet lab/Dropper bottles with secondary containment |
Shonna Dovel |
A |
|
0.01 mg/ml DAPI 4’,6-Diamidino-2-Phenylindole,Dihydrochloride |
4x1-ml aliquots |
Stored in Chem lab. Concentrated DAPI in freezer with secondary containment |
Shonna Dovel |
A |
|
Buffered Formalin (10%) |
2L |
Stored in Chem lab fume hood with secondary containment |
Shonna Dovel |
F |
|
Alkaline Lugol’s fixative (100%) |
250ml |
Stored in Chem lab refer with secondary containment |
Shonna Dovel |
F |
|
Paraformaldehyde (10%) |
.5L |
Stored in Chem lab refer with secondary containment |
Shonna Dovel |
F |
|
Proflavin (0.033%) |
250ml |
Stored in Chem lab refer with secondary containment |
Shonna Dovel |
F |
|
Sodium Thiosulfate (0.190M) |
250ml |
Stored in Chem lab refer with secondary containment |
Shonna Dovel |
F |
|
Basic Lugol’s fixative (100%) |
500ml |
Stored in Chem lab fume hood with secondary containment |
Shonna Dovel |
F |
|
Ammonium Molybdate |
75g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Ammonium Sulfate |
0.1322g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Ascorbic acid |
46g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Brij-35 (15%) |
15g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Imidazole |
8g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Copper Sulfate |
2g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
N-(1-naphthyl) ethylenediamine dihydrochl |
2g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Cadmium Coil |
3g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Oxalic acid |
100g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Sodium dodecyl sulfate |
24g |
No waste. Stored in Chem lab |
David Wolgast |
A |
|
Potassium antimony tartrate |
0.34g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Potassium Phosphate |
0.8g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Sodium chloride |
850g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Sodium Nitrite |
1.4g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Sodium hydrogen carbonate |
15g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Sodium Hydroxide |
10g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Sodium Hydroxide |
0.1L |
No waste. Stored in Chem lab |
David Wolgast |
A |
|
Ammonia Sulphate |
1L |
No waste. Stored in Chem lab |
David Wolgast |
A |
|
Sodium sulfite |
2.4g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Sulfanilamide |
20g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
O-phthalaldehyde |
4g |
No waste. Stored in Chem lab |
David Wolgast |
D |
|
Ethanol |
1500ml |
No waste. Stored in Chem lab |
David Wolgast |
F |
|
HCL (dilute 1.2N) |
2.5L |
No waste. Stored in Chem lab |
David Wolgast |
A |
|
HCL (conc. 12N) |
4L |
No waste. Stored in Chem lab |
David Wolgast |
A |
C. Chemical safety and spill response procedures
A: ACID/Bases
· Wear appropriate protective equipment and clothing during clean-up. Keep upwind. Keep out of low areas.
· Ventilate closed spaces before entering them.
· Stop the flow of material, if this is without risk. Dike the spilled material, where this is possible.
· Large Spills: Dike far ahead of spill for later disposal. Use a non-combustible material like vermiculite, sand or earth to soak up the product and place into a container for later disposal.
· Small Spills: Wipe up with absorbent material (e.g. cloth, fleece). Clean surface thoroughly to remove residual contamination.
· Never return spills in original containers for re-use.
· Neutralize spill area and washings with soda ash or lime. Collect in a non-combustible container for prompt disposal.
· J. T. Baker NEUTRASORB® acid neutralizers are recommended for spills of this product.
F: Formalin/Formaldehyde/Ethanol/Acetone
· Ventilate area of leak or spill. Remove all sources of ignition.
· Wear appropriate personal protective equipment.
· Isolate hazard area. Keep unnecessary and unprotected personnel from entering. Contain and recover liquid when possible.
· Use non-sparking tools and equipment. Collect liquid in an appropriate container or absorb with an inert material (e. g., vermiculite, dry sand, earth), and place in a chemical waste container.
· Do not use combustible materials, such as saw dust.
D: Powdered and granular chemicals
· Wear appropriate protective equipment and clothing during clean-up. Keep upwind. Keep out of low areas.
· Sweep up dry chemical and place in a doubled zip lock bag.
· If contact with water occurs, use proper neutralizing agent prior to cleanup.
· Store in sealed container to be returned and disposed by UCSD EH&S.
Inventory of Spill Kit supplies
|
Product Name |
Amount |
Chemicals it is useful against |
Amount it can clean up |
|
Chemical Spill pads |
100 |
Formaldehyde, Alcohols |
110L |
|
Uni-Safe spill binder |
14 kg |
Formaldehyde, alcohols, acids |
120L |
Note: Please see attached Appendix 1.a. detailing spill control efforts for Scripps Institution of Oceanography.
